Glucose-induced duration-dependent genome-wide DNA methylation changes in human endothelial cells.

Glucose-induced duration-dependent genome-wide DNA methylation changes in human endothelial cells. Am J Physiol Cell Physiol. 2020 May 27;: Authors: Aref-Eshghi E, Biswas S, Chen C, Sadikovic B, Chakrabarti S Abstract DNA methylation, a critical epigenetic mechanism, plays an important role in governing gene expressions during biological processes such as aging, which is well known to be accelerated in hyperglycemia (diabetes). In the present study, we investigated the effects of glucose on whole-genome DNA methylation in small (HRECs) and large (HUVECs) vessel endothelial cell (EC) lines exposed to basal or high glucose-containing media for variable lengths of time. Using the Infinium EPIC array, we obtained 773,133 CpG sites (probes) for analysis. Unsupervised clustering of the top 5% probes identified four distinct clusters within EC groups, with significant methylation differences attributed to EC types and the duration of cell culture rather than glucose stimuli alone. When comparing the ECs incubated for two days vs. seven days, hierarchical clustering analyses (methylation change >10% and false discovery rate [FDR]<0.05) identified 17,354 and 128 differentially methylated CpGs for HUVECs and HRECs, respectively. Predominant DNA hypermethylation was associated with the length of culture, and was enriched for gene enhancer elements and regions surrounding CpG shores and shelves. We identified 88 differentially methylated r...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research