Next-Generation Sequencing in High-Sensitive Detection of Mutations in Tumors: Challenges, Advances and Applications

Next-generation sequencing (NGS) technologies have come of age as preferred technologies for screening of genomic variants of pathologic and therapeutic potential. Owing to their capability for high throughput and massively parallel sequencing, they can screen for a variety of genomic changes in multiple samples simultaneously. This has made them platforms of choice for clinical testing of solid tumors and hematological malignancies. Consequently, they are increasingly replacing conventional technologies such as Sanger sequencing and Pyrosequencing, expression arrays, real-time polymerase chain reaction and fluorescence in-situ hybridization methods for routine molecular testing of tumors.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Source Type: research