Diagnostic Stewardship for Comprehensive Gastrointestinal Pathogen Panel Tests

AbstractPurpose of ReviewSyndromic gastrointestinal (GI) panels are molecular tests that evaluate stool specimens for multiple enteric pathogens simultaneously, including viruses, bacteria, and parasites. The Luminex xTAG GI panel includes 14 microbial targets and the Biofire FilmArray GI panel includes 22. The purpose of this scoping review was to describe clinical use of these two commercially available syndromic GI panels.Recent FindingsCompared with conventional testing, syndromic panels increase the number of potential pathogens identified by two- to fivefold. Syndromic panels were associated with non-reproducible results, positive results in the absence of clinical disease, and positive results for pathogens of unclear clinical significance. Syndromic panels were less likely to provide new information when performed> 72 h after hospital admission or when repeated within 4 weeks of a previous test. Process measures or clinical outcomes related to testing were infrequently reported.SummaryOutcomes-based research is needed to determine the clinical impact of syndromic GI panels. Strategies for diagnostic stewardship that may increase the utility of GI panels include (1) development of clinical criteria for testing, (2) use of multi-step testing algorithms that start with tests for the most common organisms (Clostridium difficile and norovirus), (3) selective reporting of results, (4) reporting results through a mediator such as the infection preventionist or antibiotic ...
Source: Current Infectious Disease Reports - Category: Infectious Diseases Source Type: research

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Contributors : Krista A Matthews ; Elisabeth D MartinezSeries Type : Expression profiling by high throughput sequencingOrganism : Plasmodium falciparumLittle is known about the role of the three Jumonji C (JmjC) enzymes in Plasmodium falciparum (Pf). Here, we show that JIB-04 and other established inhibitors of mammalian JmjC histone demethylases kill asexual blood stage parasites and are even more potent at blocking gametocyte development and gamete formation. In late stage parasites, JIB-04 increased levels of trimethylated lysine residues on histones, suggesting the inhibition of P. falciparum Jumonji demethylase activi...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Plasmodium falciparum Source Type: research
Publication date: Available online 11 July 2020Source: Journal of Microbiology, Immunology and InfectionAuthor(s): Cha-Shien Yen, Yhu-Chering Huang, Chih-Jung Chen, Shian-Sen Shie, Shu-Li Yang, Chung-Guei Huang, Kuo-Chien Tsao, Cheng-Hsun Chiu, Yu-Chia Hsieh, Chen-Yen Kuo, Kuan-Ying Arthur Huang, Tzou-Yien Lin
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
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Source: BMC Public Health - Category: Consumer Health News Authors: Tags: Research article Source Type: research
Fecal examination is an important component of routine companion animal wellness exams. Sensitivity and specificity of fecal examinations, however, are influenced by sample preparation methodologies and the le...
Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
de;o P. de de Sousa In low-income populations, neglected diseases are the principal cause of mortality. Of these, leishmaniasis and malaria, being parasitic, protozoan infections, affect millions of people worldwide and are creating a public health problem. The present work evaluates the leishmanicidal and antiplasmodial action of a series of twelve p-coumaric acid derivatives. Of the tested derivatives, eight presented antiparasitic activities 1–3, 8–12. The hexyl p-coumarate derivative (9) (4.14 ± 0.55 μg/mL; selectivity index (SI) = 2.72) showed the highest leishmanicidal potenc...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
Conclusion: In our experience using the PCR- Biofire assay we show that this approach is feasible and can be used safely to tailor therapy in immunocompromised patients. In all cases, E.Coli was found, most frequently STEC (Shiga-like toxin producing E. coli). Using this approach enable us to identify new pathogens causing diarrhea in immunocompromised patients, extremely quickly. These preliminary results reveal the need for more clinical experience using the Biofire FilmArray as a diagnostic test for patients with MM and other haematological malignancies, who present acutely with severe diarrhea.DisclosuresTadmor: NOVART...
Source: Blood - Category: Hematology Authors: Tags: 203. Lymphocytes, Lymphocyte Activation, and Immunodeficiency, including HIV and Other Infections Source Type: research
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Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
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Source: Journal of Microbiology, Immunology, and Infection - Category: Microbiology Authors: Tags: J Microbiol Immunol Infect Source Type: research
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Source: Transplant Infectious Disease - Category: Transplant Surgery Authors: Tags: Short Communication Source Type: research
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