A two-stage approach towards protein secondary structure classification

Abstract:Protein secondary structure (PSS) describes the local folded structures which get formed inside a polypeptide due to interactions among atoms of the backbone. Generally, globular proteins are divided into four classes, namely all-α, all-β,α + β, and  α/β. As nearly 90% of proteins fall into the said four classes, these are mostly considered for the purpose of computational classification of proteins. Classification of PSS is important for different biological functions that include protein fold recognition, tertiary structure prediction, prediction of DNA-binding sites, and reduction of the conformation search space among others. In this paper, we have proposed a machine learning –based model for secondary structure classification of proteins into four classes: all-α, all-β,α + β, and  α/β. In doing so, we have considered both sequence-based and structure-based features. At first, mutual information (MI), a filter-based feature selection method, is used to remove the redundant features, and then these selected features are used to train three different classifiers —random forest,K-nearest neighbor (KNN), and multi-layer perceptron (MLP). After that, some standard classifier combination approaches are applied to integrate the decision made by the said classifiers and it has been found that weighted product rule performs the best among all. The overall accuracies obtained using the proposed model on the four standard datasets, namely 640, 1189...
Source: Medical and Biological Engineering and Computing - Category: Biomedical Engineering Source Type: research