Front Cover: New Approaches for Absolute Quantification of Stable ‐Isotope‐Labeled Peptide Standards for Targeted Proteomics Based on a UV Active Tag

DOI:10.1002/pmic.202000007Targeted proteomics, a powerful strategy for simultaneous determination of human proteins, requires quantified stable isotope labeled (SIL) peptide standards to enable precise protein quantification. In article numberhttps://doi.org/10.1002/pmic.2020000072000007 by Karsten Schnatbaum et al., a new approach for absolute quantification of SIL peptides is reported, which eliminates the need for laborious and expensive amino acid analysis based protocols. The method relies on a UV ‐active quantification tag (Qtag) that is removed by tryptic digestion and was exemplarily applied for accurate quantification of the in‐vivo specificity of a histone methyltransferase.

Source: Proteomics - May 26, 2020 Category: Biochemistry Authors: Karsten Schnatbaum, Victor Solis ‐Mezarino, Daniil Pokrovsky, Frederike Schäfer, Dennis Nagl, Lars Hornberger, Johannes Zerweck, Tobias Knaute, Julia Avramova‐Nehmer, Mike Schutkowski, Veit Hornung, Holger Wenschuh, Moritz Carl Völker‐ Tags: Front Cover Source Type: research