Improved sensitivity using a dual target, E and RdRp assay for the diagnosis of SARS-CoV-2 infection: Experience at a large NHS Foundation Trust in the UK

We read with interest the letter from Hao et al highlighting the issues regarding the sensitivity of real time reverse-transcriptase polymerase chain reaction (RT-PCR) testing of upper respiratory tract samples for COVID19 disease [1]. Extensive RT-PCR testing by has been key to clinical decision-making, epidemiological analysis and policy development during the current severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. The majority of RT-PCR assays target the RNA-dependent RNA polymerase (RdRp), envelope protein (E) or nucleocapsid protein (N) genes [2].

Source: Journal of Infection - May 27, 2020 Category: Infectious Diseases Authors: Hayley Colton, Michael Ankcorn, Mehmet Yavuz, Leeanne Tovey, Alison Cope, Mohammad Raza, Alexander J Keeley, Amy State, Bozena Poller, Matthew Parker, Thushan I de Silva, Cariad Evans Tags: Letter to the Editor Source Type: research