Structure of TBC1D23 N-terminus reveals a novel role for rhodanese domain

by Dingdong Liu, Fan Yang, Zhe Liu, Jinrui Wang, Wenjie Huang, Wentong Meng, Daniel D. Billadeau, Qingxiang Sun, Xianming Mo, Da Jia

Members of the Tre2-Bub2-Cdc16 (TBC) family often function to regulate membrane trafficking and to control signaling transductions pathways. As a member of the TBC family, TBC1D23 is critical for endosome-to-Golgi cargo trafficking by serving as a bridge between Golgi-bound golgin-97/245 and the W ASH/FAM21 complex on endosomal vesicles. However, the exact mechanisms by which TBC1D23 regulates cargo transport are poorly understood. Here, we present the crystal structure of the N-terminus of TBC1D23 (D23N), which consists of both the TBC and rhodanese domains. We show that the rhodanese domain is unlikely to be an active sulfurtransferase or phosphatase, despite containing a putative catalytic site. Instead, it packs against the TBC domain and forms part of the platform to interact with golgin-97/245. Using the zebrafish model, we show that impacting golgin-97/245-binding, but not the putative catalytic site, impairs neuronal growth and brain development. Altogether, our studies provide structural and functional insights into an essential protein that is required for organelle-specific trafficking and brain development.

Source: PLoS Biology: Archived Table of Contents - May 25, 2020 Category: Biology Authors: Dingdong Liu Source Type: research