Comprehensive analysis of the secreted proteome of adult < i > Necator americanus < /i >  hookworms

by Jayden Logan, Mark S. Pearson, Srikanth S. Manda, Young-Jun Choi, Matthew Field, Ramon M. Eichenberger, Jason Mulvenna, Shivashankar H. Nagaraj, Ricardo T. Fujiwara, Pedro Gazzinelli-Guimaraes, Lilian Bueno, Vitor Mati, Jeffrey M. Bethony, Makedonka Mitreva, Javier Sotillo, Alex Loukas The human hookwormNecator americanus infects more than 400 million people worldwide, contributing substantially to the poverty in these regions. Adult stageN.americanus live in the small intestine of the human host where they inject excretory/secretory (ES) products into the mucosa. ES products have been characterized at the proteome level for a number of animal hookworm species, but until now, the difficulty in obtaining sufficient liveN.americanus has been an obstacle in characterizing the secretome of this important human pathogen. Herein we describe the ES proteome ofN.americanus and utilize this information along with RNA Seq data to conduct the first proteogenomic analysis of a parasitic helminth, significantly improving the available genome and thereby generating a robust description of the parasite secretome. The genome annotation resulted in a revised prediction of 3,425 fewer genes than initially reported, accompanied by a significant increase in the number of exons and introns, total gene length and the percentage of the genome covered by genes. Almost 200 ES proteins were identified by LC-MS/MS with SCP/TAPS proteins, ‘hypothetical’ proteins and proteases among the most abun...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research