Detection of African swine fever virus in cell culture and wild boar tissues using a commercially available monoclonal antibody.

In this study two sensitive tests were developed for the detection of the p72 major capsid protein of ASFV both in cell culture with an immunocytochemical (IC) and in tissue samples with an immunohistochemical (IHC) method using a commercially available mouse monoclonal antibody (clone 1BC11). The IC test was able to detect the virus at high virus dilutions in cell culture and the IHC test indicated the presence of ASFV in all formalin-fixed and paraffin-embedded tissue samples collected from two wild boars. The reported IC and IHC methods were found to be useful ancillary laboratory tests for research purposes and for the diagnosis of acute ASF. PMID: 32450150 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research