In Vitro Methods to Simulate Pollution and Photo-Pollution Exposure in Human Skin Epidermis.

In Vitro Methods to Simulate Pollution and Photo-Pollution Exposure in Human Skin Epidermis. Methods Mol Biol. 2020 May 24;: Authors: Misra N, Marrot L Abstract Tissue homeostasis of an individual is a finely orchestrated phenomenon that ensures integrity and steady state in health. Emerging evidence indicates that the environment, especially ambient air pollution, has a lasting impact on this equilibrium (Beelen et al., Lancet 383:785-795, 2014). Environmental pollution consists of diverse entities, namely, particulate matter (PM 2.5, PM 10), ozone, and UV rays, among others (Heroux et al., Int J Public Health 60:619-627, 2015). Understandably, skin epidermis is the first and the most exposed tissue to such a wide range of substances and bears the assault. Previous studies have established that exposure to atmospheric pollution aggravates several skin disorders as, for instance, eczema, acne, lentigines or macules, and wrinkles (Araviiskaia et al., J Eur Acad Dermatol Venereol 33:1496-1505, 2019). While pollutants can interact with skin surface, contamination of deep skin by particulate matter (either ultrafine particles or by some polycyclic aromatic hydrocarbon (PAH) moieties) is also highly probable, particularly because PAH were detected in blood and inside the cortex of hair (Guo et al., Sci Total Environ 427-428:35-40, 2012; Palazzi et al., Environ Int 121:1341-1354, 2018). Importantly, concentrations of contaminant PAH in the...
Source: Reproductive Biology - Category: Reproduction Medicine Authors: Tags: Methods Mol Biol Source Type: research