Complement component C1q plays a critical role in VLRA/VLRC-mediated immune response.

Complement component C1q plays a critical role in VLRA/VLRC-mediated immune response. Dev Comp Immunol. 2020 May 21;:103750 Authors: Li J, Ma Q, Liu H, Song X, Pang Y, Su P, Sun F, Gou M, Lu J, Shan Y, Liu X, Li Q, Han Y Abstract In jawless vertebrates, the lamprey complement component C1q (LC1q) acts as a lectin and activates lamprey complement component C3 (LC3) in association with mannose-binding lectin (MBL)-associated serine protease (MASP) via the lectin pathway. Furthermore, LC1q may interact with variable lymphocyte receptor B (VLRB) in a complex with antigens and mediate the activation of LC3, leading to cytolysis. In the present study, we found, for the first time, that LC1q plays a critical role in VLRA/VLRC-mediated immune response. Escherichia coli, Shigella flexneri, Aeromonas hydrophila, Pseudomonas plecoglossicida, Aeromonas allosaccharophila, P. luteola, Brevundimonas diminuta, and Bacillus cereus were isolated from infected Lampetra morii in our laboratory and identified using the 16s rRNA method. A. hydrophila was confirmed as a rapidly spreading lethal pathogen in the larvae of L. morii and was used in subsequent immune stimulation experiments. The results of real-time quantitative polymerase chain reaction (Q-PCR) and immunofluorescence analyses indicated that the RNA and protein expression levels of LC1q were upregulated following exposure to 107 cfu/mL of A. hydrophila, compared to the levels of the naïve gr...
Source: Developmental and Comparative Immunology - Category: Allergy & Immunology Authors: Tags: Dev Comp Immunol Source Type: research