GSE149975 Multimodal and multisensory coding in the Drosophila larval peripheral gustatory center

Contributors : G L Maier ; Johannes Bues ; Marjan Bio čanin ; Felix Meyenhofer ; Clarisse Brunet Avalos ; Jae Y Kwon ; Bart Deplancke ; Simon G SprecherSeries Type : Expression profiling by high throughput sequencingOrganism : Drosophila melanogasterThe ability to evaluate food palatability is innate in all animals from insects to mammals, ensuring their survival. The external taste organ at the periphery in Drosophila larvae is composed of only few sensory neurons but displays discrimination between a wide range of chemicals as well as a high level of complexity in receptor expression and cell response profile. Taking advantage of this architecturally simple but functionally complex sensory system, we tackled dissection of taste coding at organ level with cellular resolution. We combined whole-organ calcium imaging and single-cell transcriptomics to map physiological properties and molecular features of individual sense neurons. In line with taste multimodality, about one third of gustatory sense neurons responded to multiple tastants. Reinforcing on the peripheral integration of signals, we observed neuronal deactivation events within simultaneous neighboring responses, submitting the probability of inter-cellular communication through electrical coupling. Furthermore, we identified neurons responding to both mechanical and taste stimulation, indicating potential multisensory integration. On a molecular level, chemosensory cells show heterogeneity in neuromodulator ex...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Drosophila melanogaster Source Type: research