Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19.

Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19. J Virol Methods. 2020 May 20;:113888 Authors: Campos GS, Sardi SI, Falcao MB, Belitardo EMMA, Rocha DJPG, Rolo CA, Menezes AD, Pinheiro CS, Carvalho RH, Almeida JPP, Aguiar ERGR, Pacheco LGC Abstract Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus. PMID: 32445875 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research