Lactobacillus plantarum-12 Exopolysaccharides Have Anti-Biofilm Activity Against Shigella flexneri.

In this study, the effects of Lactobacillus plantarum-12 exopolysaccharides (L-EPS) and S. flexneri exopolysaccharides (S-EPS) on the S. flexneri CMCC51574 biofilm formation were investigated. The results showed that L-EPS could decrease the polysaccharides production in the extracellular polymeric matrix of S. flexneri, and inhibit the biofilm formation of S. flexneri L-EPS could decrease the minimum biofilm elimination concentration (MBEC) of the antibiotics against S. flexneri biofilm and inhibit S. flexneri adhesion and invasion to the HT-29 cell monolayers, which might be ascribed to the S. flexneri biofilm disturbance by L-EPS. While the S-EPS exhibited the exactly opposite effects compared to L-EPS. The monosaccharide composition analysis showed that L-EPS was composed of mannose, glucuronic acid, galactosamine, glucose, galactose and xylose, with the molar ratio of 32.26: 0.99: 1.79: 5.63: 0.05: 4.07, and the S-EPS was composed of mannose, glucuronic acid, galactosamine, glucose, galactose, with the molar ratio of 25.43: 2.28: 7.13: 5.35. The L-EPS was separated to the neutral polysaccharide L-EPS 1-1 and the acidic polysaccharide L-EPS 2-1 by the ion exchange chromatography and gel chromatography. The L-EPS 2-1 exerted higher anti-biofilm activity than the L-EPS 1-1. The anti-biofilm activity of L-EPS might be associated with its structure.Importance:S. flexneri is a wide-spread food-borne pathogen causing food contamination and responsible for food poisoning outbrea...

Source: Applied and Environmental Microbiology - May 21, 2020 Category: Microbiology Authors: Song Y, Sun M, Feng L, Liang X, Song X, Mu G, Tuo Y, Jiang S, Qian F Tags: Appl Environ Microbiol Source Type: research