Homology-dependent recombination of large synthetic pathways into E. coli genome via λ-Red and CRISPR/Cas9 dependent selection methodology

Metabolic engineering frequently needs genomic integration of many heterologous genes for biosynthetic pathway assembly. Despite great progresses in genome editing for the model microorganism Escherichia coli, th...

Source: Microbial Cell Factories - May 24, 2020 Category: Microbiology Authors: Buli Su, Dandan Song and Honghui Zhu Tags: Research Source Type: research