Effect of Nonthermal Processing on Human Milk Bactericidal Activity Against Escherichia coli
This study was performed to assess the effects of nonthermal processing on bactericidal activity against Escherichia coli in human milk. Milk samples obtained from the Regional Human Milk Bank in Warsaw at Holy Family Hospital were processed by HoP, irradiated with ultraviolet-C (UV-C) for 5, 10, and 15 minutes (6720 J/L each minute), subjected to 2 variations of high-pressure processing (HPP): 450 MPa for 15 minutes and 200 MPa for 10 minutes + 400 MPa for 10 min, with a 10-minutes break. The samples were then evaluated by a bactericidal assay (raw untreated human milk was used as a control). The bactericidal capacity after HoP was preserved in 12.1% of samples, showing a significant reduction in bactericidal properties compared with in raw milk (P 0.05). Nonthermal methods of human milk treatment better preserve the bactericidal capacity compared with holder pasteurisation. Those alternative technologies to HoP can be proposed after further investigation for milk processing for Human Milk Banks facilities.
Authors: Iwamuro M, Tanaka T, Okada H PMID: 32641661 [PubMed - as supplied by publisher]
CONCLUSIONS Our results showed that 8-oxoG clearance in L. braziliensis is important to avoid misincorporation during DNA replication after oxidative stress generated by H2O2. PMID: 32638832 [PubMed - in process]
Conclusions: The food safety conditions and the quality of ice cubes at manufacturing premises in Binh Phuoc Province were not acceptable. Hygiene deficiencies in ice producing and handling processes, inadequate knowledge of staff, inadequate food safety management practices, and hygiene conditions of the premises were important factors in producing contaminated ice products. We recommend more staff training and crediting the staff when performing adequate practices, paying attention to broken floors or difficulty in cleaning, structural deficiency, and ensuring regular monitoring of premises. This study adds a special int...
CONCLUSIONS: In summary, microbial EVs demonstrated the potential in their use as novel biomarkers for AD diagnosis. Therefore, future work should investigate larger case and control groups with cross-sectional or longitudinal clinical data to explore the utility and validity of serum microbiota EV-based AD diagnosis. PMID: 32638560 [PubMed]
Publication date: Available online 9 July 2020Source: LWTAuthor(s): Seung-Hae Gwak, Jin-Hee Kim, Se-Wook Oh
Contributors : Nicole L Mancini ; Timothy S Jayme ; Matthew L Workentine ; Derek M McKaySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-seq was performed to interogate the transcriptional changes in response to infection with Adherent Invasive E. coli and a control, non-invasive E. coli
Contributors : Nicole L Mancini ; Matthew L Workentine ; Derek M McKaySeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-seq was performed to interogate the transcriptional changes in response to infection with Adherent Invasive E. coli and a control, non-invasive E. coli
Publication date: Available online 9 July 2020Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Victor Hladkyi Toledo, Tania Mateus Yoshimura, Saulo Toledo Pereira, Carlos Eduardo Castro, Fabio Furlan Ferreira, Martha Simões Ribeiro, Paula Silvia Haddad
In a wide range of organisms, from bacteria to humans, numerous proteins have to be posttranslationally acylated to become biologically active. Bacterial repeats in toxin (RTX) cytolysins form a prominent group of proteins that are synthesized as inactive protoxins and undergo posttranslational acylation on ε-amino groups of two internal conserved lysine residues by co-expressed toxin-activating acyltransferases. Here, we investigated how the chemical nature, position, and number of bound acyl chains govern the activities of Bordetella pertussis adenylate cyclase toxin (CyaA), Escherichia coli α-hemolysin (Hl...