Revisiting the window of opportunity for co-transcriptional splicing in budding yeast.

We reported previously that, in budding yeast, transcription rate affects both the efficiency and fidelity of pre-mRNA splicing, especially of ribosomal protein transcripts. Here, we report that the majority of ribosomal protein transcripts with non-consensus 5' splice sites are spliced less efficiently when transcription is faster, and more efficiently with slower transcription. These results support the "window of opportunity" model, and we suggest a possible mechanism to explain these findings.
PMID: 32439718 [PubMed - as supplied by publisher]

Source: RNA - May 20, 2020 Category: Genetics & Stem Cells Authors: Aslanzadeh V, Beggs JD Tags: RNA Source Type: research