Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice.

Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice.
Exp Brain Res. 2020 May 18;:
Authors: Dong X, Zhang X, Wang F, Liu N, Liu A, Li Y, Wei L, Chen F, Yuan S, Zhang K, Hou S, Jiao Q, Hu Q, Guo C, Wu T, Wei S, Shen H
Abstract
Epilepsy is a common neurological disorder characterized by recurrent epileptic seizures. The cause of most cases of epilepsy is unknown. Although changes of calcium events in a single brain region during seizures have been reported before, there have been few studies on relations between calcium events of two different brain regions and epileptic behaviors in freely moving mice. To analyze calcium events simultaneously recorded in hippocampal CA1 (CA1) and primary motor cortex M1 (M1), and to explore their relations to various epileptic behaviors in freely moving epileptic models. Epileptic models were induced by Kainic acid (KA), a direct agonist of glutamatergic receptor, on adult male C57/BL6J mice. Calcium events of neurons and glia in CA1 and M1 labeled by a calcium indicator dye were recorded simultaneously with a multi-channel fiber photometry system. Three typical types of calcium events associated with KA-induced seizures were observed, including calcium baseline-rising, cortical spreading depression (CSD) and calcium flashing with a steady rate. Our results showed that the calcium baseline-rising occ...

Source: Experimental Brain Research - May 17, 2020 Category: Neuroscience Authors: Dong X, Zhang X, Wang F, Liu N, Liu A, Li Y, Wei L, Chen F, Yuan S, Zhang K, Hou S, Jiao Q, Hu Q, Guo C, Wu T, Wei S, Shen H Tags: Exp Brain Res Source Type: research