UCLA scientists alter genes of innate immune cells with DNA-snipping tool

A UCLA research team has successfully used  the powerful gene-editing tool known as CRISPR-Cas9 to alter the DNA of mature innate immune cells, some of the body’s first responders to infections. These blood cells have been notoriously difficult to genetically engineer in the past.While the work was carried out in mice, the ability to  modify the gene expression of these cells could one day allow clinicians to better harness the power of the immune system in the fight against cancer and autoimmune disease.The study, published in the journal Cell Reports, was led by senior author  Tim O’Sullivan, an assistant professor of microbiology, immunology and molecular genetics and a member of the Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA.“We’ve figured out a way to genetically edit cells that researchers have had a lot of difficulty with in the past,” said O’Sullivan, who is also a member of the UCLA Jonsson Comprehensive Cancer Center. “The real impact will be if we can one day take cells from patients’ blood, edit them using this approach and put them back in the same patient as an immunotherapy.”Unlike the adaptive immune system, which relies on  the production of antibodies that target specific molecules present on viruses, bacteria, and other pathogens, the innate immune system is the body’s first...
Source: UCLA Newsroom: Health Sciences - Category: Universities & Medical Training Source Type: news

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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Conclusion and Future Perspectives This review illustrates our current knowledge of USP7, including its source and characterization, structure, binding partners and substrates in various biological processes. Besides, how USP7 regulates various aspects of a cell under both normal and pathological states are elaborated in detail. As the processes of ubiquitination and deubiquitination are extremely dynamic and context-specific, a series of studies have linked USP7 to different cancers. The biology, particularly the immune oncology mechanisms, reveal that USP7 inhibitors would be useful drugs, thus it is vital to develop hi...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Conclusion and Perspectives Being exquisitely regulated by “writers,” “erasers,” and “readers,” additional repelled proteins or miRNAs, m6A modification relates to nearly any step of mRNA metabolism, as well as ncRNA processing and circRNA translation. There is compelling evidence suggesting that m6A modification is especially critical in a variety of pathologic and physiologic immune responses including T cell homeostasis and differentiation, inflammation, and type I interferon production. Further results have indicated that aberrancies of interferon and Th17 frequencies in systemic lu...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Conclusions Lymphatic vessels, like blood vessels, are a highly interactive surface for cells of the immune system, and through the use of chemokines and their receptors can coordinate key interactions. These pathways can control the entry and function of particular immune subsets in a number of pathological conditions. Nonetheless LECs have distinct patterns of chemokine secretion and expression of chemokine receptors that distinguish them from the blood vessel system and mediate distinct roles and responses. The abundance and diversity of the chemokine family point to the likelihood that a plethora of novel chemokine fu...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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