Detoxification of Aflatoxin B1 contaminated maize using human CYP3A4.

Detoxification of Aflatoxin B1 contaminated maize using human CYP3A4. J Microbiol Biotechnol. 2020 May 13;: Authors: Yamada M, Hatsuta K, Niikawa M, Imaishi H Abstract Aflatoxin B1 (AFB1), a mycotoxin produced by Aspergillus flavus (A. flavus). AFB1 is reported to have high thermal stability and is not decomposed by heat treatment during food processing processes. Therefore, in this study, knowing that AFB1 is metabolized by cytochrome P450 (CYP), our aim was to develop a method to detoxify A. flavus contaminated maize, under normal temperature and pressure, using E. coli expressing human CYP3A4. First, the metabolic activity of AFB1 by recombinant human CYP3A4 was evaluated. As a result, we confirmed that recombinant human CYP3A4 metabolizes 98% of AFB1. Next, we found that aflatoxin Q1, a metabolite of AFB1 was no longer mutagenic. Furthermore, we revealed that about 50% of the AFB1 metabolic activity can be maintained for 3 months when E. coli expressing human CYP3A4 is freeze-dried in the presence of trehalose. Finally, we found that 80% of AFB1 in A. flavus contaminated maize was metabolized by E. coli expressing human CYP3A4 in the presence of surfactant triton X-405 at a final concentration of 10% (v/v). From these results, we conclude that AFB1 in A. flavus contaminated maize can be detoxified under normal temperature and pressure by using E. coli expressing human CYP3A4. PMID: 32423188 [PubMed - as supplied by publis...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research