Gene analysis of major signalling pathways regulated by gonadotropins in human ovarian granulosa tumour cells (KGN) †.

Gene analysis of major signalling pathways regulated by gonadotropins in human ovarian granulosa tumour cells (KGN)†. Biol Reprod. 2020 May 19;: Authors: Tremblay PG, Sirard MA Abstract The female reproductive function largely depends on timing and coordination between follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Even though it was suggested that these hormones act on granulosa cells via shared signalling pathways, mainly protein kinases A, B, and C (PKA, PKB, PKC), there is still very little information available on how these signalling pathways are regulated by each hormone to provide such differences in gene expression throughout folliculogenesis. To obtain a global picture of the principal upstream factors involved in PKA, PKB, and PKC signalling in granulosa cells, human granulosa-like tumour cells (KGN) were treated with FSH or specific activators (forskolin, SC79, PMA) for each pathway to analyze gene expression with RNA seq technology. Normalization and cut-offs (FC 1.5, p ≤ 0.05) revealed 3,864 differentially expressed genes between treatments. Analysis of major upstream regulators showed that PKA is a master kinase of early cell differentiation as its activation resulted in the gene expression profile that accompanies granulosa cell differentiation. Our data also revealed that the activation of PKC in granulosa cells is also a strong differentiation signal that could control "advanced" differentiat...
Source: Biology of Reproduction - Category: Reproduction Medicine Authors: Tags: Biol Reprod Source Type: research