Long-Read Nanopore Sequencing Validated for Human Leukocyte Antigen Class I Typing in Routine Diagnostics

Matching of human leukocyte antigen (HLA) gene polymorphisms by high-resolution DNA sequence analysis is the gold standard for determining compatibility between patient and donor for hematopoietic stem cell transplantation. Single-molecule sequencing (PacBio or MinION) is a newest (third) generation sequencing approach. MinION is a nanopore sequencing platform, which provides long targeted DNA sequences. The long reads provide unambiguous phasing, but the initial high error profile prevented its use in high-impact applications, such as HLA typing for HLA matching of donor and recipient in the transplantation setting.

Source: Journal of Molecular Diagnostics - April 13, 2020 Category: Pathology Authors: Benedict M. Matern, Timo I. Olieslagers, Mathijs Groeneweg, Burcu Duygu, Lotte Wieten, Marcel G.J. Tilanus, Christina E.M. Voorter Tags: Regular article Source Type: research