Effect of Decellularization Parameters on the Efficient Production of Kidney Bioscaffolds.

This study evaluated the effects of decellularization parameters specifically SDS concentration (0.25%, 0.625%, and 1.0%wt/vol), flowrate (15, 30, and 45 mL/min), and sonicator power (0, 60, and 120 W) on the length of time needed to produce acellular and intact bioscaffolds. Decellularization was carried out by perfusing SDS to the renal artery of the cadaveric porcine kidney while exposed to sonication treatment. Results showed that a significant decrease in decellularization time was observed in producing acellular scaffold when perfusion decellularization was coupled with sonication. In addition, SDS concentration, SDS flowrate, and sonicator power had significant effects on the decellularization time while only sonicator power had a significant effect on the microarchitecture integrity of the scaffold. Lastly, H&E results showed that the produced bioscaffold showed complete cell removal with only minimal to moderate disruptions on the microarchitecture of the kidney. PMID: 32418019 [PubMed - as supplied by publisher]
Source: Applied Biochemistry and Biotechnology - Category: Biochemistry Authors: Tags: Appl Biochem Biotechnol Source Type: research