Two Glycosyl 1-Phosphate Polymers and Teichulosonic Acid from Glutamicibacter protophormiae VKM Ac-2104 T Cell Wall

AbstractTwo glycosyl 1-phosphate polymers containing monoglycosyl 1-phosphate, -6)- α-D-Glcp-(1-P., and diglycosyl 1-phosphate, -6)- α-D-GalpNAc-(1 →6)-α-D-GlcpNAc-(1-P., in the repeating unit were identified in the cell wall ofGlutamicibacter protophormiae VKM Ac-2104T (formerly,Arthrobacter protophormiae). The structures of these polymers were described for the first time in prokaryotes. Teichulosonic acid, the third identified polymer, with 3-deoxy-D-glycero- α-D-galacto-non-2-ulopyranosonic acid (Kdn) and β-D-glucopyranose residues in the main chain, →6)-β-D-Glcp-(1 →8)-α-Kdn-(2→, has been previously detected in a number of actinobacteria. The structures of these glycopolymers were established based on the results of chemical analysis and one-dimensional1H,13C, and31P NMR spectroscopy using two-dimensional homonuclear (1H,1H COZY, TOCSY, ROESY) and heteronuclear (1H,13C HSQC, HSQC-TOCSY, HMBC, and1H,31P HMBC) techniques.

Source: Biochemistry (Moscow) - April 30, 2020 Category: Biochemistry Source Type: research