Effect of Luminal Flow on Doming of mpkCCD Cells in a 3D Perfusable Kidney Cortical Collecting Duct Model.

Effect of Luminal Flow on Doming of mpkCCD Cells in a 3D Perfusable Kidney Cortical Collecting Duct Model. Am J Physiol Cell Physiol. 2020 May 13;: Authors: Rein JL, Heja S, Flores D, Carrisoza-Gaytán R, Lin NYC, Homan KA, Lewis JA, Satlin LM Abstract The cortical collecting duct (CCD) of the mammalian kidney plays a major role in the maintenance of total body electrolyte, acid/base and fluid homeostasis by tubular reabsorption and excretion. The mammalian CCD is heterogeneous, comprised of Na+-absorbing principal cells (PCs) and acid-base transporting intercalated cells (ICs). Perturbations in luminal flow rate alter hydrodynamic forces to which these cells in the cylindrical tubules are exposed. However, most studies of tubular ion transport have been performed in cell monolayers grown on or epithelial sheets affixed to a flat support, since analysis of transepithelial transport in native tubules by in vitro microperfusion requires considerable expertise. Here, we report on the generation and characterization of an in vitro, perfusable 3D kidney CCD model (3D CCD), in which immortalized mouse PC-like mpkCCD cells are seeded within a cylindrical channel embedded within an engineered extracellular matrix and subjected to luminal fluid flow. We find that a tight epithelial barrier composed of differentiated and polarized PCs forms within 1 week. Immunofluorescence microscopy reveals the apical epithelial Na+ channel ENaC and basolate...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research