An Innovator Support Material for Tyrosinase Immobilization: Antimony-Doped Tin Oxide Thin Films (ATO-TF).

An Innovator Support Material for Tyrosinase Immobilization: Antimony-Doped Tin Oxide Thin Films (ATO-TF). Appl Biochem Biotechnol. 2020 May 11;: Authors: Türkhan A, Kaya ED, Koçyiğit A Abstract Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectroscopy (FTIR). The optimum pH of the immobilized tyrosinase (tyrosinase-ATO-TF) was 6.0, and the optimum temperature was found to be 30 °C. In the presence of catechol substrate of immobilized enzymes, Km and Vmax values were determined as 0.34 mM and 312.5 U/cm2 min, respectively. The thermal stabilities of the immobilized tyrosinase at 4 °C and 30 °C were investigated for 20, 40, and 60 min. At these temperatures and time intervals, the immobilized tyrosinase was found to be highly stable. The pH stability of the immobilized tyrosinase enzymes was incubated for 24, 48, 72, and 96 h at 4 °C temperature. The enzyme activity was determined under optimum conditions, and the activity of the immobilized tyrosinase enzymes exhibited various values for the range of pH 3.0-8.0. The storage stability of the immobilized tyrosinase enzymes at 4 °C was investigated, and 45.72% of the initial activity was maintained at the en...
Source: Applied Biochemistry and Biotechnology - Category: Biochemistry Authors: Tags: Appl Biochem Biotechnol Source Type: research