Detection and Quantification of Chimeric Antigen Receptor Transgene Copy Number by Droplet Digital PCR versus Real-Time PCR
In this study, key performance parameters of the ddPCR and real-time PCR methods were assessed, including linearity, detection range, the lower limit of detection, repeatability, reproducibility, and accuracy, using a series of gradient diluted standards and clinical peripheral blood samples from CAR T-cell patients.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Yaoyao Lou, Caixia Chen, Xiaolu Long, Jia Gu, Min Xiao, Di Wang, Xiaoxi Zhou, Tongjuan Li, Zhenya Hong, Chunrui Li, Jianfeng Zhou, Liting Chen Tags: Regular article Source Type: research