Depletion of macrophages slows the early progression of renal injury in obese Dahl salt-sensitive leptin receptor mutant rats.

Depletion of macrophages slows the early progression of renal injury in obese Dahl salt-sensitive leptin receptor mutant rats. Am J Physiol Renal Physiol. 2020 May 11;: Authors: Poudel B, Shields CA, Brown AK, Ekperikpe US, Johnson TD, Cornelius DC, Williams JM Abstract Recently, we reported that obese Dahl salt-sensitive leptin receptor mutant (SSLepRmutant) rats display progressive renal injury. The current study demonstrated that the early development of renal injury in the SSLepRmutant strain is associated with an increase in the renal infiltration of macrophages compared to lean SS rats. We also examined whether depletion of macrophages with clodronate would reduce the early progression of renal injury in the SSLepRmutant strain. Four week-old SS and SSLepRmutant rats were treated with either vehicle (PBS) or clodronate (50 mg/kg, i.p., twice/week) for 4 weeks. While the administration of clodronate did not reduce renal macrophage infiltration in SS rats, clodronate decreased macrophages in the kidneys of SSLepRmutant rats by > 50%. Interestingly, clodronate significantly reduced plasma glucose, insulin, and triglyceride levels and markedly improved glucose tolerance in SSLepRmutant rats. Treatment with clodronate had no effect on the progression of proteinuria or renal histopathology in SS rats. In the SSLepRmutant strain, proteinuria was markedly reduced during the first 2 weeks of treatment (159±32 vs. 303±52 mg/day, res...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research