Sputum Cell-free DNA: Valued Surrogate Sample for Detection of EGFR Mutation in Patients with Advanced Lung Adenocarcinoma

Sputum is common cytological sample type, but its potential use in EGFR mutation detection in patients with lung cancer is not fully evaluated. An improved sputum cfDNA extraction method was established in this study, and applied super-amplification refractory mutation system (SuperARMS) to detect the EGFR mutation status in sputum cfDNA. The sputum sediments were used for cytology evaluation. The study included 102 lung adenocarcinoma patients, in which 65 (63.7%) patients were positive for EGFR mutations in tumor samples.

Source: Journal of Molecular Diagnostics - May 10, 2020 Category: Pathology Authors: Zheng Wang, Lin Zhang, Lin Li, Xiaoguang Li, Yan Xu, Mengzhao Wang, Li Liang, Peng Jiao, Yuanming Li, Shurong He, Jun Du, Lei He, Min Tang, Mingjun Sun, Li Yang, Jing Di, Guanshan Zhu, Hong Shi, Dongge Liu Source Type: research