Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study.

Marked epithelial to mesenchymal transition in surgical margins of oral cancer-an in vitro study. Oncol Lett. 2020 Jun;19(6):3743-3750 Authors: Lazarevic M, Milosevic M, Jelovac D, Milenkovic S, Tepavcevic Z, Baldan F, Suboticki T, Toljic B, Trisic D, Dragovic M, Damante G, Milasin J Abstract Epithelial to mesenchymal transition (EMT) is a feature of several types of human cancer, including oral squamous cell carcinoma (OSCC). In the present study, tumor and margin cell cultures obtained from patients with OSCC were used to determine the expression patterns of certain EMT-associated markers, including vimentin, α-smooth muscle actin, SLUG and SNAIL. In addition, other EMT-associated features, including clonal, proliferative and migratory potential were compared between the two cell types. Cell cultures were generated from tumor and margin tissue samples from 6 patients and cultured up to the fifth passage. EMT marker expression was assessed by reverse transcription-quantitative PCR. Cell proliferation, colony formation and scratch wound healing assays were conducted to characterize the two cell types in terms of proliferation rates, clonality and motility. All of the studied markers were expressed in tumor and margin cells. Although no significant differences were noted with regard to the aforementioned markers, their expression tended to be higher in margin cultures than in tumor cultures. The expressions of the EMT markers were al...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research