Long non-coding RNA DSCAM-AS1 upregulates USP47 expression through sponging miR-101-3p to accelerate osteosarcoma progression.

In this study, our findings proved that DSCAM-AS1 was highly expressed in OS cells. DSCAM-AS1 knockdown suppressed cell proliferation, migration and invasion, as well as induced cell apoptosis in OS. Additionally, DSCAM-AS1 suppression inactivated the Wnt/β-catenin pathway. Moreover, researches of molecular mechanism confirmed that DSCAM-AS1 could function as a sponge for miR-101-3p and Ubiquitin-specific peptidase 47 (USP47) was the target gene of miR-101-3p. Furthermore, a negative relationship between miR-101-3p and DSCAM-AS1 or USP47 was discovered. Rescue assays suggested that DSCAM-AS1 regulated OS progression through binding with miR-101-3p to modulate USP47 expression. Finally, we found that AKT/mTOR signal pathway might be a possible principle in mediating DSCAM-AS1 in OS. Taken together, DSCAM-AS1 accelerated OS progression via miR-101-3p/USP47 axis, which might present a new potential therapeutic treatment for OS. PMID: 32379981 [PubMed - as supplied by publisher]
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research