Interleukin-31 interaction with inflammasome: A promising diagnostic and prognostic panel for early sepsis identification in critically ill patients

Purpose: This case-control study aimed to evaluate the ability to use a panel of IL-31, IL-1s and NLRP3 to differentiate sepsis from systemic inflammatory response syndrome (SIRS) and to predict septic shock. Method(s): Serum levels of IL-31, IL-1s and NLRP3 were measured by ELISA in 149 participants; 38 with sepsis, 51 with SIRS, 30 with septic shock and 30 healthy controls. Result(s): Lower levels of IL-31 were found in sepsis (10.21 +/- 4.34 pg/ml) compared to SIRS (16.74 +/- 3.18 pg/ml) and to controls with the lowest levels detected in septic shock (6.26 +/- 2.72 pg/ml). IL-1s and NLRP3 levels were higher in sepsis (54.99 +/- 14.11 pg/ml and 9.93 +/- 2.38 ng/ml) compared to SIRS (27.8 +/- 6.94 pg/ml and 4.86 +/- 1.33 ng/ml) with the highest levels seen in septic shock (125.1 +/- 32.79 pg/ml and 19.43 +/- 6.48 ng/ml) respectively. IL-31 discriminated sepsis in patients showing SIRS with 80% sensitivity and 70% specificity and, identified septic shock with 78.6% sensitivity and 60.3% specificity. IL-1s identified sepsis from SIRS with 93.3% and 83.3% specificity. NLRP3 discriminated sepsis from SIRS with 94.5% sensitivity and 93.3% specificity. And, with sensitivity 99.1% and 90.1% and specificity 98.9% and 80% IL-1s and NLRP3 could respectively define septic shock. A panel of combined markers provided 100% sensitivity and specificity. The three biomarkers proved to be independent prognostic biomarkers. At 95% CI, IL-31 hazard ratio (HR) was 0.716, p = 0.001; IL-1beta HR w...

Source: Current Awareness Service for Health (CASH) - May 6, 2020 Category: Consumer Health News Source Type: news