Effects of Colonization of Gnotobiotic Swiss Webster Mice with Helicobacter bilis.

Effects of Colonization of Gnotobiotic Swiss Webster Mice with Helicobacter bilis. Comp Med. 2020 Apr 29;: Authors: Whary MT, Wang C, Ruff CF, DiVincenzo MJ, Labriola C, Ge L, Feng Y, Ge Z, Bakthavatchalu V, Muthupalani S, Horwitz BH, Fox JG Abstract Helicobacter bilis (Hb) causes hepatitis in some strains of inbred mice. The current study confirmed that Hb directly causesportal hepatitis in outbred gnotobiotic Swiss Webster (SW) mice, as we previously reported for conventional SW mice. Hb monoassociated SW mice also developed mild enterocolitis, expanded gut-associated lymphoid tissue (GALT), and tertiarylymphoid tissue in the lower bowel. At 1 and 10 mo after infection, Hb-induced GALT hyperplasia exhibited well-organized,ectopic germinal centers with increased mononuclear cell apoptosis, MHC class II antigen presentation, and pronouncedendothelial venule formation, consistent with features of tertiary lymphoid tissue. In the lower bowel, Hb induced mainlyB220+ cells as well as CD4+IL17+, CD4+IFNγ+, and CD4+FoxP3+ regulatory T cells and significantly increased IL10 mRNAexpression. This gnotobiotic model confirmed that Hb causes portal hepatitis in outbred SW mice but stimulated GALTwith an antiinflammatory bias. Because Hb had both anti- and proinflammatory effects on GALT, it should be considered a'pathosymbiont provocateur' and merits further evaluation in mouse models of human disease. PMID: 32349859 [PubMed - as suppli...
Source: Comparative Medicine - Category: Zoology Authors: Tags: Comp Med Source Type: research