An increase in AMPK/e-NOS signaling and attenuation of MMP-9 may contribute to remote ischemic perconditioning associated neuroprotection in rat model of focal ischemia.

An increase in AMPK/e-NOS signaling and attenuation of MMP-9 may contribute to remote ischemic perconditioning associated neuroprotection in rat model of focal ischemia. Brain Res. 2020 Apr 27;:146860 Authors: Parray A, Ma Y, Alam M, Akhtar N, Salam A, Mir F, Qadri S, Sajitha VP, Priyanka J, Kamran S, Winship IR, Shuaib A Abstract Remote ischemic perconditioning (RIPerC) results in collateral enhancement and a reduction in middle cerebral artery occlusion (MCAO) induced ischemia. RIPerC likely activates multiple metabolic protective mechanisms, including effects on matrix metalloproteinases (MMPs) and protein kinases. Here we explore if RIPerC improves neuroprotection and collateral flow by modifying the activities of MMP-9 and AMPK/e-NOS. Age matched adult male Sprague Dawley rats were subjected to MCAO followed two hours later by RIPerC (3 cycles of 15 minutes ischemia). Animals were euthanized 24 hours post-MCAO. Haematoxylin and Eosin (H&E) staining 24 h post-MCAO revealed a significant (p< 0.02) reduction in the infarction volume in RIPerC treated animals (24.9 ± 5.4%) relative to MCAO controls (42.5 ± 4.2, %). TUNEL staining showed a 42.6% reduction in the apoptotic cells with RIPerC treatment (p <0.01). Immunoblotting in congruence with RT-PCR and Zymography showed that RIPerC significantly reduced MMP-9 expression and activity in RIPerC+ MCAO group compared to MCAO group (218.3 ±19.1% vs. 148.9 ± 12.05% (p< 0...
Source: Brain Research - Category: Neurology Authors: Tags: Brain Res Source Type: research