Non-invasive Diagnosis of Invasive Pulmonary Aspergillosis by Use of Biomarkers in Exhaled Breath Condensate
Conditions: Aspergillosis Pneumonia; Pneumocystis Pneumonia Interventions: Diagnostic Test: Aspergillus specific proteins in exhaled breath condensate (EBC); Diagnostic Test: Pneumocystis jirovecii DNA in exhaled breath condensate (EBC) Sponsors: Inger Lise Gade; Aarhus University Hospital Recruiting
CONCLUSIONS: The estimated prevalence for probable Aspergillus infection in this geographically dispersed multicentre UK cohort indicates that this condition should be considered when investigating patients with suspected ventilator-associated pneumonia, including patient groups not previously recognised to be at high risk of aspergillosis. PMID: 32609533 [PubMed - as supplied by publisher]
Semin Respir Crit Care Med DOI: 10.1055/s-0040-1702210Nowadays, reports in the literature support that patients with severe chronic obstructive pulmonary disease (COPD) are at higher risk to develop invasive pulmonary aspergillosis (IPA). However, the interpretation of Aspergillus-positive cultures from the airways in critically ill COPD is still a challenge. Indeed, as the patient could be merely colonized, tissue samples are required to ascertain IPA diagnosis but they are rarely obtained before death. Consequently, diagnosis is often only suspected on the basis of a combination of three elements: clinical characteristic...
The objective of this study was to investigate the clinical characteristics and outcomes of patients with MAC-LD positive for APAb.Methods: We retrospectively analyzed patients who were newly diagnosed with MAC-LD between 2007 and 2014 in our hospital and checked for APAb within one month of diagnosis.Results: We enrolled 131 patients in this study. Of these patients, 20 (15.3%) tested positive for APAb at diagnosis of MAC-LD. APAb-positive patients were more frequently male (70.0% vs. 37.8%, P = 0.013) and more frequently had pulmonary emphysema (60.0% vs. 13.5%, P
Conclusions: Overall results of surgery in this cohort were good and number of complications was low. Therapy with antifungals was diverse. Surgical treatment of aspergilloma can be life-saving for patients suffering of haemoptysis, and patients with restricted disease and well-preserved pulmonary capacity may benefit from surgery. Careful patient selection is crucial. PMID: 31737315 [PubMed]
ConclusionsChronic and allergic forms of aspergillosis occurred in 17.5% of STAT3-deficient patients, mostly in lung cavities. Almost half had recurrences, despite prolonged antifungal treatment and/or surgery.
Conclusions A serum Aspergillus-specific IgG test is a valuable tool for the serologic diagnosis of CPA and SAIA, while an Aspergillus-specific IgM test is only modestly specific for the diagnosis of SAIA. Overall, the Aspergillus-specific IgG test is more valuable compared to IgM for the treatment duration monitoring.
In this study they also showed PTX3 localized in NETs formed after neutrophil activation (5). Proteomics analysis revealed that PTX3 forms complexes with two anti-microbial proteins [azurocidin (AZU1) and myeloperoxidase (MPO)] associated to NETs (30). More recently, PTX3 localization in NETs has been confirmed, and the colocalization with AZU1 and MPO has been defined more accurately (31). Further investigation will be needed to understand the involvement of PTX3 interaction with AZU1 and MPO in their antibacterial role during NET formation. Regulation of Complement Activation PTX3 interaction with microorganisms is not...
ConclusionChronic and allergic forms of aspergillosis occurred in 17.5% of STAT3 deficient patients, mostly in lung cavities. Almost half had recurrences, despite prolonged antifungal treatment and/or surgery.