Gentianella acuta prevents acute myocardial infarction induced by isoproterenol in rats via inhibition of galectin-3/TLR4/MyD88/NF- кB inflammatory signalling

AbstractGentianella acuta (G.  acuta), as a folk medicine, was used to treat heart disease by the Ewenki people in Inner Mongolia. However, the effect ofG.  acuta on acute myocardial infarction (AMI) is not clear. To explore the mechanisms ofG.  acuta on isoproterenol (ISO)-induced AMI, rats were administeredG.  acuta for 28  days, then injected intraperitoneally with ISO (85 mg/kg) on days 29 and 30. An electrocardiogram helped to evaluate the myocardial injury. Serum lactate dehydrogenase (LDH), creatinine kinase (CK) and aspartate aminotransferase (AST) levels were evaluated, and haematoxylin eosin, Masson’s tric hrome staining and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining were used to detect myocardial histological changes. Radioimmunoassay was used to measure serum tumour necrosis factor alpha (TNFα) and interleukin (IL)-6. An enzyme-linked immunosorbent assay kit was used to analyse serum galectin-3 (Gal-3) levels. Immunohistochemistry, Western blotting and reverse transcription polymerase chain reaction were used to examine relevant molecular events. The results revealed that pre-treatment withG.  acuta decreased the elevation in the ST segment; reduced serum LDH, CK and AST levels; alleviated cardiac structure disorder; and reduced inflammatory infiltration, abnormal collagen deposition and cardiomyocyte apoptosis that were induced by ISO. Furthermore, pre-treatment withG.  acuta inhibited serum Gal-3 levels and Gal-3 ex...
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research