Cx30.2 deletion causes imbalances in testicular Cx43, Cx46 and Cx50 and insulin receptors. Reciprocally, diabetes/obesity alters Cx30.2 in mouse testis.

Cx30.2 deletion causes imbalances in testicular Cx43, Cx46 and Cx50 and insulin receptors. Reciprocally, diabetes/obesity alters Cx30.2 in mouse testis.
Am J Physiol Regul Integr Comp Physiol. 2020 Apr 29;:
Authors: Pelletier RM, Layeghkhavidaki H, Kumar NM, Vitale ML
Abstract
Cx30.2 protein content and localisation were assessed during development. An account of Cx30.2, Cx43, Cx46 and Cx50, and insulin receptor (IR) responses to Cx30.2, Cx46 or Cx50 deficiency in mouse interstitial tissue- (ITf) and seminiferous tubule-enriched fractions (STf) is given. The impact of high glucose/insulin on Cx30.2 was investigated in spontaneously diabetic and obese db/db and ob/ob mouse testis and anterior pituitary (AP). Cx30.2 labelled contacts in vascular endothelial and Leydig cells and Sertoli cell junctions in stage V-VII. Cx30.2 expression is regulated differently in the interstitium and tubules. 30kDa Cx30.2 levels peaked by 28 days in ITf and by 14 days in STf. In STf, deleting Cx30.2 decreased Cx43 and Cx50 whereas deleting Cx50 downregulated Cx30.2. The opposite occurred in ITf. In STf, deleting Cx30.2 upregulated Cx46 except the full length reciprocally, deleting Cx46 upregulated Cx30.2. In ITf, Cx30.2 deficiency upregulated full length and phosphorylated Cx46 whereas deleting Cx46 downregulated 48-50kDa Cx30.2. The db/db and ob/ob mouse ITf, STf and AP showed imbalanced Cx30.2 levels. 135kDa IRα levels declined in Cx30.2-/- and Cx50-/...

Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - April 28, 2020 Category: Physiology Authors: Pelletier RM, Layeghkhavidaki H, Kumar NM, Vitale ML Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research