T-cell Epitope-based Vaccine design for Nipah Virus by Reverse Vaccinology approach.

T-cell Epitope-based Vaccine design for Nipah Virus by Reverse Vaccinology approach. Comb Chem High Throughput Screen. 2020 Apr 27;: Authors: Krishnamoorthy PKP, Subasree S, Arthi U, Imam M, Gowda C, Prasanna Dr Abstract Nipah virus (NiV) is a zoonotic virus of the paramyxovirus family that sporadically breaks out from livestock and spread in human through breathing resulting in indication of encephalitis syndrome. In the current study, T cell epitopes with the NiV W protein antigens were predicted. Modelling of unavailable 3D structure of W protein followed by docking studies of respective Human MHC - class I and MHC - class II alleles predicted was carried out for the highest binding rates. In the computational analysis, epitopes were assessed for immunogenicity, conservation, and toxicity analysis. T - cell based vaccine development against NiV was screened for eight epitopes of Indian - Asian origin. Two epitopes SPVIAEHYY, LVNDGLNII, have been screened and selected for further docking study based on toxicity and conservancy analyses. These epitopes showed a significant score of -1.19 kcal/mol, 0.15 kcal/mol with HLA- B*35:03, HLA- DRB1 * 07:03, allele - Class I and Class II using AutoDock. These two peptides predicted by reverse vaccinology approach are likely to induce immune response mediated by T - cells. Simulation using GROMACS has revealed LVNDGLNII epitope forms more stable complex with HLA molecule and will be useful in ...
Source: Combinatorial Chemistry and High Throughput Screening - Category: Chemistry Authors: Tags: Comb Chem High Throughput Screen Source Type: research