A fluorescence method to visualize the nuclear boundary by the lipophilic dye DiI.

A fluorescence method to visualize the nuclear boundary by the lipophilic dye DiI. Biosci Biotechnol Biochem. 2020 Apr 23;:1-4 Authors: Miyazaki K, Yano KI, Saitoh H Abstract Here, we describe a procedure to fluorescently contrast the nuclear boundary using the lipophilic carbocyanine dye DiI in cultured human cells. Our procedure is simple and is applicable to detect nuclear boundary defects, which may be relevant to studies on nuclear envelope dynamics, micronuclei formation and cancer biology.Abbreviations: DiI: 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate; DiO: 3,3'-dioctadecyloxacarbocyanine perchlorate; NE: nuclear envelope; RanBP2: Ran-binding protein 2/Nucleoporin 358. PMID: 32326840 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/32326840?dopt=Abstract

Source: Bioscience, Biotechnology, and Biochemistry - April 22, 2020 Category: Biochemistry Authors: Miyazaki K, Yano KI, Saitoh H Tags: Biosci Biotechnol Biochem Source Type: research