Lentivirus ‑mediated CD44s expression increases human basal cell carcinoma cell resistance against vismodegib.

Lentivirus‑mediated CD44s expression increases human basal cell carcinoma cell resistance against vismodegib. Oncol Rep. 2020 Mar 06;: Authors: Ren J, Ma X, Tu C, Li Z Abstract High drug resistance, which is usually mediated by drug resistance‑associated genes, is a characteristic of human tumours. CD44s, an ATP‑binding cassette multidrug resistance transporter, is expressed in a variety of human cancers. In the present study, the effect of CD44s expression was investigated on BCC resistance against vismodegib. Lentiviral vectors were constructed to allow efficient CD44s expression. Cell clones expressing the CD44s construct were selected and expanded and then identified using qRT‑PCR and western blotting. A lentiviral vector containing a blank sequence was used as a control. Cellular growth capacity and cell sensitivity to vismodegib were detected by MTT and Transwell assays, respectively. BCC cell growth was evaluated in vivo with a transplanted BCC nude mouse model. The cell clones expressing CD44s at high levels were identified by qRT‑PCR and western blotting, and the difference in the cell proliferation rate between these cells and LV‑CON BCC cells was assessed by growth curve analysis. The in vitro study revealed that treatment with vismodegib decreased BCC cell growth and migration; however, these effects were reversed by LV‑CD44s overexpression. The in vivo study revealed that BCC tumour growth was significantly increa...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

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Source: Progress in Lipid Research - Category: Lipidology Source Type: research
Publication date: Available online 15 July 2020Source: The Annals of Thoracic SurgeryAuthor(s): Kristen E. Rhodin, Vignesh Raman, Oliver K. Jawitz, Soraya L. Voigt, Norma E. Farrow, David H. Harpole, Betty C. Tong, Thomas A. D’Amico
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
Authors: Li X, Gao L, Zheng L, Shi J, Ma J Abstract The role of autophagy in tumor development is complicated. More signaling mechanisms of bone morphogenetic protein 4 (BMP4) involving in hepatocellular carcinoma (HCC) are needed to be clarified. The present study aimed to identify whether BMP4 contributes to the regulation of autophagy in the progression of HCC. We found that increased BMP4 expression was significantly correlated with TNM stage and metastasis. BMP4 treatment promoted HCC cells invasion and induced autophagy. Blocking autophagy by 3-MA or silenced Beclin1 attenuated BMP4-induced autophagy and cell...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Authors: Zhou Y, Chi Y, Bhandari A, Xia E, Thakur PC, Qu J, Wang O, Zhang X Abstract BACKGROUND: Placental-Cadherin (CDH3), a cell adhesion molecule, is associated with the function of cells to bind with other cells and the extracellular matrix (ECM). CDH3 is highly expressed in many malignancies, and has been proved it could be a serum marker to monitor colorectal cancer, but the CDH3 expression levels in thyroid cancer is still not clear. In this article, we will illuminate the correlation between CDHs expression and thyroid cancer. MATERIALS AND METHODS: We analyzed the level of CDH3 expression in 60 pair of...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
This study provides a theoretical basis for the improvement of QOL in BC patients. PMID: 32655829 [PubMed]
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Authors: Dai T, Deng M, Ye L, Liu R, Lin G, Chen X, Li H, Liu W, Yang Y, Chen G, Wang G Abstract The gamma-glutamyl transpeptidase to platelet ratio (GPR) has been reported as a non-invasive parameter for evaluating hepatic fibrosis and cirrhosis. However, only a few of studies investigated the relationship between GPR and liver cancer. Here, we sought to clarify the prognostic value of GPR as well as its combination with fibrinogen in patients with HBV-related hepatocellular carcinoma (HCC). We performed a retrospective study using data collected from 302 HCC patients, and evaluated the association between GPR, fi...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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