miR-16-5p is upregulated by amyloid β deposition in Alzheimer's disease models and induces neuronal cell apoptosis through direct targeting and suppression of BCL-2.

miR-16-5p is upregulated by amyloid β deposition in Alzheimer's disease models and induces neuronal cell apoptosis through direct targeting and suppression of BCL-2. Exp Gerontol. 2020 Apr 19;:110954 Authors: Kim YJ, Kim SH, Park Y, Park J, Lee JH, Kim BC, Song WK Abstract Alzheimer's disease (AD) is the most common form of dementia with irreversible neurodegeneration. Accumulation of amyloid beta (Aβ) in the brain is considered to be a major cause of neuronal cell death in AD, but the neurotoxic mechanism of Aβ is not yet fully understood. Here, we focused on the role of microRNAs (miRNAs) in Aβ-induced neuronal cell death. In microarray and RT-qPCR analysis of plasma miRNAs obtained from 5 familiar AD mutations (5xFAD) and wild-type (WT) mice of various ages, miR-16-5p showed a significant age-related change that was accompanied by neuronal cell death in the brain tissue of 5xFAD mice. In addition, increased miR-16-5p was prominent near Aβ plaque-deposition sites in 5xFAD mouse brains. Aβ treatment induced miR-16-5p upregulation and apoptosis in primary cultured mouse cortical neurons and the SH-SY5Y human neuroblastoma cell line. In silico analysis and reporter gene assays indicated that miR-16-5p directly targets the mRNA encoding the anti-apoptotic factor, B cell lymphoma-2 (BCL-2), in the neuronal cell line. Overexpression of miR-16-5p in SH-SY5Y cells downregulated BCL-2 expression and induced apoptosis. These results...
Source: Experimental Gerontology - Category: Geriatrics Authors: Tags: Exp Gerontol Source Type: research

Related Links:

We report a new approach for the development of multitargeted therapeutics for Alzheimer ’s disease (AD) based on dual targeting of monomeric tau and biogenesis of microRNA-146a. Compound MG-1102 displayed superior... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - Category: Chemistry Authors: Source Type: research
Cancer patients are a population at high risk of contracting COVID-19 and, also of developing severe complications due to the infection, which is especially true when they are undergoing immunosuppressive treatment. Despite this, they had still to go to hospital to receive chemotherapy during lockdown. In this context, we have evaluated the psychological status of onco-hematological outpatients receiving infusion and not deferrable anti-neoplastic treatment for lymphoproliferative neoplasms, with the aim of both measuring the levels of post-traumatic symptoms, depression, and anxiety during the pandemic and also of investi...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Publication date: Available online 9 July 2020Source: Journal of the Formosan Medical AssociationAuthor(s): Chao-Hsien Hung, Guang-Uei Hung, Cheng-Yu Wei, Ray-Chang Tzeng, Pai-Yi Chiu
Source: Journal of the Formosan Medical Association - Category: General Medicine Source Type: research
Publication date: Available online 8 July 2020Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Hai-Ming An, Da-Rong Huang, Hua Yang, Xin-Guang Liu, Jing Du, Li Yi, Chao-Ran Li, Han-Qing Pang, Run-Zhou Liu, Chao Peng, Ping Li, Wen Gao
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research
CONCLUSIONS: This study addresses a gap in current research and holds important implications for enhancing care planning participation by residents with severe dementia. CLINICAL IMPLICATIONS: Residents with severe dementia can successfully participate in activity preference discussions without proxy participation. Residents may comprehend interview questions better when provided in a supported format. PMID: 32633699 [PubMed - as supplied by publisher]
Source: Clinical Gerontologist - Category: Geriatrics Tags: Clin Gerontol Source Type: research
Publication date: Available online 9 July 2020Source: Revista Española de Medicina Nuclear e Imagen Molecular (English Edition)Author(s): I. Cabrera Veloz, J. Uña Gorospe, A. Allende Riera, C. Cardenas Negro
Source: Revista Espanola de Medicina Nuclear e Imagen Molecular - Category: Nuclear Medicine Source Type: research
Authors: Qi F, Jiang X, Tong T, Chang H, Li RX Abstract OBJECTIVE: To explore the influences of micro ribonucleic acid (miR)-204 on the rats with diabetic retinopathy by regulating the expressions of B-cell lymphoma 2 (Bcl-2) and sirtuin 1 (SIRT1). MATERIALS AND METHODS: A total of 36 Sprague-Dawley rats were randomly assigned into normal group (n=12), model group (n=12), and miR-204 mimics group (n=12). No treatment was performed in the normal group, the diabetic retinopathy model was established in model group, and miR-204 mimics were administered for intervention after modeling in the inhibitor group. After ...
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Authors: Zhao GZ, Niu YQ, Li ZM, Kou D, Zhang L Abstract OBJECTIVE: The aim of this study was to explore the effect of micro ribonucleic acid (miR)-200c on the proliferation and apoptosis of Wilms tumor cells, and to further elucidate its potential mechanisms. PATIENTS AND METHODS: Reverse Transcription-Polymerase Chain Reaction (RT-PCR) was used to detect the expression level of miR-200c in cancer tissues and adjacent normal tissues of 20 patients with Wilms tumor. Human primary Wilms tumor cells were taken as research objects, and were divided into Control group and miR-200c mimic group. In miR-200c mimic gro...
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Conditions:   Ann Arbor Stage I Extranodal Marginal Zone Lymphoma of Mucosa-Associated Lymphoid Tissue;   Ann Arbor Stage II Extranodal Marginal Zone Lymphoma of Mucosa-Associated Lymphoid Tissue;   Extranodal Marginal Zone Lymphoma Intervention:   Other: Radiation Therapy Sponsors:   M.D. Anderson Cancer Center;   National Cancer Institute (NCI) Active, not recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Conditions:   Acute Myeloid Leukemia;   Acute Lymphoid Leukemia;   Myelodysplastic Syndromes;   Myeloproliferative Disorders;   Chronic Myeloid Leukemia;   Myelofibrosis;   Multiple Myeloma;   Malignant Lymphoma Interventions:   Drug: MDG1021 dose 1;   Drug: MDG1021 dose 2;   Drug: MDG1021 dose 3;   Drug: MDG1021 optimal dose Sponsor:   Medigene AG Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
More News: Alzheimer's | Brain | Dementia | Genetics | Geriatrics | Lymphoma | Neuroblastoma | Neurology | Neuroscience | Science