Forward programming of pluripotent stem cells to neurons.

Forward programming of pluripotent stem cells to neurons. Curr Mol Med. 2020 Apr 21;: Authors: Gu J, Cromer B, Sumer H Abstract Pluripotent stem cells (PSCs) are powerful tools for studying developmental biology and neuronal diseases. Conventional differentiation protocols require several intermediate states and different culture conditions, inefficiently generating mixed subtypes of neuronal cells with immature characteristics. Direct programming of PSCs by forced expression of neuronal transcription factors has shown rapid cell fate determination with high purity as it can bypass sequential developmental steps that traditional culture requires. In this review, we focus on neuronal differentiation from PSCs to specific subtypes by various transcription factors. Furthermore, the potential applications and limitations of this novel technology are discussed. PMID: 32316890 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/32316890?dopt=Abstract

Source: Current Molecular Medicine - April 20, 2020 Category: Molecular Biology Authors: Gu J, Cromer B, Sumer H Tags: Curr Mol Med Source Type: research