Impurity Profiling HPTLC Method involving Assay of Essential Human Micronutrient Niacin with Eco Scale Assessment.

Impurity Profiling HPTLC Method involving Assay of Essential Human Micronutrient Niacin with Eco Scale Assessment. Biomed Chromatogr. 2020 Apr 19;:e4858 Authors: Naguib IA, Draz ME, Abdallah FF Abstract Recently, the special attention of analytical society is being paid to reducing reliance on hazardous chemicals in various analytical methods. By embracing this concept, an eco-friendly HPTLC method is developed as an alternative for the conventional HPLC method for determination of an essential human micronutrient, Niacin (NIA) which was introduced in the market to improve the lipid profile of patients. Furthermore, the proposed HPTLC method is capable of determination of the structurally related impurities of NIA such as pyridine-2,5-dicarboxylic acid (PDC), isonicotinic acid (ISO), pyridine (PYR), and 5-ethyl-2-methylpyridine (EMP) which have nephrotoxic and hepatotoxic effects. The separation of this challenging mixture was achieved on HPTLC sheets using mixture of ethyl acetate: ethanol: ammonia solution (6: 4: 0.05, by volume) then the dried plates were scanned at 254 nm. The analytical Eco-scale assessment protocol was utilized to assess the greenness profile of the presented method and compare it to the reported HPLC method. The suggested method was found to be greener with regard to the consumption of solvents and the yielding of waste. This outcome encourages us to implement the described method safely for analysis of NIA ph...
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research