GSE146771 Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted therapies in colon cancer

Contributors : Lei Zhang ; Ziyi Li ; Katarzyna M Skrzypczynska ; Qiao Fang ; Wei Zhang ; Sarah A O ’Brien ; Yao He ; Lynn Wang ; Qiming Zhang ; Aeryon Kim ; Ranran Gao ; Jessica Orf ; Tao Wang ; Deepali Sawant ; Jiajinlong Kang ; Dev Bhatt ; Daniel Lu ; Chi-Ming Li ; Aaron Rapaport ; Kristy Perez ; Yingjiang Ye ; Shan Wang ; Xueda Hu ; Xianwen Ren ; Wenjun Ouyang ; Zhanlong Shen ; Jackson G Egen ; Zemin Zhang ; Xin YuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensSingle cell RNA sequencing (scRNA-seq) is a powerful tool for defining cellular diversity in tumors, but its application towards dissecting mechanisms underlying immune-modulating therapies is scarce. We performed scRNA-seq analyses on immune and stromal populations from colorectal cancer patients, identifying specific macrophage and conventional dendritic cell (cDC) subsets as key mediators of cellular cross-talk in the tumor microenvironment. Defining comparable myeloid populations in mouse tumors enabled characterization of their response to myeloid-targeted immunotherapy. Treatment with anti-CSF1R preferentially depleted macrophages with an inflammatory signature but spared macrophage populations that in mouse and human expresses pro-angiogenic/tumorigenic genes. Treatment with a CD40 agonist antibody preferentially activated a conventional dendritic cell population and increased Bhlhe40+ Th1-like cells and CD8+ memory T cells. Our comprehensive a...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research