Target-Enriched Next-Generation Sequencing Reveals Differences between Primary and Secondary Ovarian Tumors in Formalin-Fixed, Paraffin-Embedded Tissue

Publication date: Available online 26 December 2014 Source:The Journal of Molecular Diagnostics Author(s): Stijn Crobach , Dina Ruano , Ronald van Eijk , Gert Jan Fleuren , Ivonne Minderhout , Ronelle Snowdowne , Carli Tops , Tom van Wezel , Hans Morreau Differentiating primary endometrioid or mucinous ovarian tumors from secondary ovarian tumors can be challenging. We compared somatic mutation profiles of primary and secondary ovarian cancers to investigate if these profiles can help diagnose ovarian tumors. Cancer-related genes (n = 115) were screened by target-enriched next-generation sequencing in formalin-fixed, paraffin-embedded tumor tissue from 43 primary endometrioid and mucinous ovarian carcinomas and 28 proven colorectal cancer metastases to the ovary. Results were validated by high-resolution melting curve analysis and Sanger sequencing. TP53, NOTCH1, PIK3CA, and FAT4 and APC, TP53, KRAS, and FAT4 mutations were the most common in the primary ovarian tumors and ovarian colorectal cancer metastases, respectively. An inactivating APC mutation was found in 4.7% of primary ovarian tumors (2 of 43; 95% CI, 1.6%–10.9%). In contrast, inactivating APC mutations were identified in 71% of colorectal cancer metastases (20 of 28; 95% CI, 55%–88%) (P < 0.001; sensitivity: 71.4%, 95% CI, 51.1%–86.0%; specificity: 95.4%, 95% CI, 82.9%–99.1%). Loss of heterozygosity and APC promoter hypermethylation did not differ significantly between the primary an...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research