MicroRNA-518-3p suppresses cell proliferation, invasion and migration in colorectal cancer via targeting TRIP4.

MicroRNA-518-3p suppresses cell proliferation, invasion and migration in colorectal cancer via targeting TRIP4. Biochem Cell Biol. 2020 Apr 16;: Authors: Yang H, Ren J, Bai Y, Jiang J, Xiao S Abstract MicroRNA (miR)-518-3p has been shown to function as a tumor suppressor. The present study was undertaken to investigate the effects of miR-518-3p in colorectal cancer (CRC). The miR-518-3p mimic, mimic negative control (NC), miR-518-3p inhibitor, inhibitor-NC, ShRNA-TRIP4 and ShRNA-NC vectors were transfected into SW480 cells using Lipofectamine 2000. Cell viability was detected using CCK 8. The clone-formation, invasive and migratory capacities were separately assessed by plate clone formation, Transwell and wound healing assays. Relative mRNA and protein levels were detected using RT qPCR and western blot, respectively. The target gene thyroid hormone receptor interactor 4 (TRIP4) of miR-518-3p was identified and further verified using dual luciferase reporter assay. Compared with normal people, miR-518-3p was decreased and TRIP4 was elevated significantly in patients with CRC. Following transfections with miR-518-3p mimic or ShRNA-TRIP4, cell viability decreased in a time‑dependent manner. The cloning efficiency, wound closure rate and the number of invading cells were much lower than those in the corresponding NC and control groups. miR-518-3p overexpression or silence of TRIP4 significantly down-regulated MMP‑2/‑9 expression....
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research