Viruses, Vol. 12, Pages 450: A Mouse Model of Oropharyngeal Papillomavirus-Induced Neoplasia Using Novel Tools for Infection and Nasal Anesthesia

Viruses, Vol. 12, Pages 450: A Mouse Model of Oropharyngeal Papillomavirus-Induced Neoplasia Using Novel Tools for Infection and Nasal Anesthesia Viruses doi: 10.3390/v12040450 Authors: Andrea Bilger Renee E. King Josh P. Schroeder Jared T. Piette Louis A. Hinshaw Andrew D. Kurth Ronnie W. AlRamahi Matthew V. Barthel Ella T. Ward-Shaw Darya Buehler Kristyn S. Masters Susan L. Thibeault Paul F. Lambert Human head and neck cancers that develop from the squamous cells of the oropharynx (Oropharyngeal Squamous Cell Carcinomas or OPSCC) are commonly associated with the papillomavirus infection. A papillomavirus infection-based mouse model of oropharyngeal tumorigenesis would be valuable for studying the development and treatment of these tumors. We have developed an efficient system using the mouse papillomavirus (MmuPV1) to generate dysplastic oropharyngeal lesions, including tumors, in the soft palate and the base of the tongue of two immune-deficient strains of mice. To maximize efficiency and safety during infection and endoscopy, we have designed a nose cone for isoflurane-induced anesthesia that takes advantage of a mouse’s need to breathe nasally and has a large window for oral manipulations. To reach and infect the oropharynx efficiently, we have repurposed the Greer Pick allergy testing device as a virus delivery tool. We show that the Pick can be used to infect the epithelium of the soft palate and the base of the tongue of m...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research