Investigation of in vivo anti-inflammatory and anti-angiogenic attributes of coumarin-rich ethanolic extract of Melilotus indicus

AbstractInflammation and angiogenesis are two major contributors to tumourigenesis.Melilotus indicus is traditionally used as an anti-inflammatory agent. The current study was designed to investigate the anti-inflammatory and anti-angiogenic properties of ethanolic extract ofM. indicus (Miet) whole plant and its marker compound (coumarin) using a series of in vivo methods. Extraction by maceration was adopted to prepare ethanolic extract. Phytochemical compounds present inMiet were investigated using both qualitative and quantitative methods. In vivo safety profile ofMiet was investigated in behavioural studies. Four acute oedema models such as carrageenan, serotonin, histamine-induced paw oedema and xylene-induced ear oedema, and chronic formaldehyde-induced paw oedema model were employed to explore the anti-inflammatory potential ofMiet. Chorioallantoic chick membrane assay (CAM) was performed to explore anti-angiogenic potential ofMiet. Histopathological evaluations were conducted to access improvement in skin texture of paws. TNF- α ELISA kit was used to study effects of treatment on serum levels of TNF-α. Extraction by maceration resulted in formation of greenish coloured semisolid extract with a high coumarin content. In vivo toxicological studies revealed LD50 ofMiet was greater than 8000  mg/kg. Data of acute inflammatory models depicted significant (p <  0.05) inhibition of oedema inMiet, coumarin and standard (piroxicam/indomethacin) treated groups. 750  ...
Source: Inflammopharmacology - Category: Drugs & Pharmacology Source Type: research