Concordance in molecular genetic analysis of tumour tissue, plasma, and exhaled breath condensate samples from lung cancer patients

Aim. Lung adenocarcinoma is characterized by poor prognosis and short survival rates. Therefore,
tools to identify the tumoural molecular structure and guide effective diagnosis and therapy
decisions are essential. Surgical biopsies are highly invasive and not conducive for patient
follow-up. To better understand disease prognosis, novel non-invasive analytic methods are needed.
The aim of the present study is to identify the genetic mutations in formalin-fixed
paraffin-embedded (FFPE) tissue, plasma, and exhaled breath condensate (EBC) samples by
next-generation sequencing and evaluate their utility in the diagnosis and follow-up of patients
with lung adenocarcinoma. Method. FFPE, plasma, and EBC samples were collected from 12 lung
adenocarcinoma patients before treatment. DNA was extracted from the specimens using an Invitrogen
PureLink Genomic DNA Kit according to the manufacturer ’s instructions. Amplicon-based sequencing was
performed using Ion AmpliSeq Colon ...

Source: Journal of Breath Research - April 13, 2020 Category: Respiratory Medicine Authors: Asl ı Tetik Vardarli, Levent Pelit, Ceyda Aldag, Korcan Korba, Caglar Celebi, Tugberk Nail Dizdas, Umut Can Uzun, Eda Tayfur, Ayca Aykut, Haydar Soydaner Karakus, Ertan Baysal, Ozlem Goksel, Fusun Pelit, Femin Yalcin, Fatma Nil Ertas, Yasemin Basbinar, A Source Type: research