Resuscitation with pegylated-carboxyhemoglobin preserves renal cortical oxygenation and improves skeletal muscle microcirculatory flow during endotoxemia.

Resuscitation with pegylated-carboxyhemoglobin preserves renal cortical oxygenation and improves skeletal muscle microcirculatory flow during endotoxemia. Am J Physiol Renal Physiol. 2020 Apr 13;: Authors: Guerci P, Ergin B, Kandil A, Ince Y, Heeman P, Hilty MP, Bakker J, Ince C Abstract PEGylated-carboxyhemoglobin (PEGHbCO), which has carbon monoxide-releasing properties, plasma expansion and oxygen-carrying properties, may improve both skeletal microcirculatory flow and renal cortical microcirculatory partial oxygen pressure (CµPO2) and subsequently limit endotoxemia-induced acute kidney injury (AKI). Anesthetized, ventilated Wistar albino rats (n=44) underwent endotoxemic shock. CµPO2 was measured in exposed kidneys using a phosphorescence-quenching method. Rats were randomly assigned to five groups: (1) unresuscitated lipopolysaccharide (LPS); (2) Ringer's acetate (LPS+RA); (3) LPS+RA+0.5µg.kg.-1min-1 norepinephrine (NE); (4) LPS+RA+PEGHbCO (320mg.kg-1); (5) LPS+RA+PEGHbCO+NE. Total volume was 30ml.kg-1 in each group. A time control animal group was used. Skeletal muscle microcirculation was assessed by handheld intravital microscopy. Kidney immunohistochemistry and myeloperoxidase-stained leukocytes in glomerular and peritubular areas were analyzed. Endotoxemia-induced histological damage was assessed. Plasma levels of interleukin-6, heme oxygenase-1, malondialdehyde, and syndecan-1 were assessed by enzyme-linked immunosorben...
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research